Peter Dutton has told Malcolm Turnbull he has the majority support of the Liberal party room and wants to challenge him again.
The ex-Home Affairs Minister lost a challenge 48 votes to 35 on Tuesday but now wants to have another go at toppling his leader.
"A few minutes ago I spoke with Malcolm Turnbull to advise him I believed the majority of the party room no longer supported his leadership," Mr Dutton tweeted on Thursday.
"Accordingly, I asked him to convene a party room meeting at which I would challenge for the leadership of the Parliamentary Liberal Party."
Two more federal government ministers have formally resigned to support Mr Dutton's bid.
Assistant ministers Michael Sukkar and Zed Seselja have refused the prime minister's offer to remain on in their positions, throwing their weight behind Mr Dutton ahead of a second leadership challenge.